Caspase 7 antibody (N-Term)

Catalog No. ABIN6990521

The Rabbit Polyclonal anti-Caspase 7 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Caspase 7 in samples from Human, Mouse and Rat.

Quick Overview for Caspase 7 antibody (N-Term) (ABIN6990521)

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 7 Antibody

Binding Specificity: AA 30-80, N-Term

Specificity: Depending on cell lines or tissues used, other cleavage products may be observed.

Purification: Caspase-7 Antibody is affinity chromatography purified via peptide column.

Immunogen: Caspase-7 antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human Caspase-7. The immunogen is located within amino acids 30 - 80 of Caspase-7.

Isotype: IgG

Application Details

Application Notes: Casp-7 antibody can be used for the detection of Caspase-7 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Caspase-7 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Caspase-7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: Caspase-7

Background: Caspase-7 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-7 is an executioner caspase that was identified based on its homology with caspases 1 and 3, as well as the C. elegans cell death protein CED-3. Alternative splicing of Caspase-7 mRNA results in the production of 3 distinct isoforms. Caspase-7 activity can be directly inhibited by XIAP expression.

Gene ID: 840

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity