MCL-1 antibody (AA 110-160)

Catalog No. ABIN6990528

This anti-MCL-1 antibody is a Rabbit Polyclonal antibody detecting MCL-1 in WB, ELISA and ICC. Suitable for Human.

Quick Overview for MCL-1 antibody (AA 110-160) (ABIN6990528)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-MCL-1 Antibody

Binding Specificity: AA 110-160

Specificity: Detects isoforms Mcl-1L and Mcl-1S

Purification: Mcl-1 Antibody is Ion exchange chromatography purified.

Immunogen: Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the center of human Mcl-1. The immunogen is located within amino acids 110 - 160 of Mcl-1.

Isotype: IgG

Application Details

Application Notes: Mcl-1 antibody can be used for detection of isoforms Mcl-1L and Mcl-1S by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Mcl-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Mcl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: Mcl-1

Background: Mcl-1 Antibody: Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least three distinct isoforms designated Mcl-1L, Mcl-1S and Mcl-1ES. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.

Gene ID: 4170

NCBI Accession: NP_068779

UniProt: Q07820

Pathways: MAPK Signaling