RHEB antibody (N-Term)

Catalog No. ABIN6990533

The Rabbit Polyclonal anti-RHEB antibody has been validated for WB and ELISA. It is suitable to detect RHEB in samples from Human, Mouse and Rat.

Quick Overview for RHEB antibody (N-Term) (ABIN6990533)

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHEB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RHEB Antibody

Binding Specificity: N-Term

Specificity: Post-translationally modified Rheb is sometimes observed at higher molecular weight.

Purification: Rheb Antibody is affinity chromatography purified via peptide column.

Immunogen: Rabbit Rheb polyclonal antibody was raised against a 14 amino acid synthetic peptide from the amino terminus of human Rheb. The immunogen is located within the first 50 amino acids of Rheb.

Isotype: IgG

Application Details

Application Notes: Rheb antibody can be used for the detection of Rheb by Western blot at 2 and 4 μ,g/mL.

Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rheb Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Rheb antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for RHEB

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Alternative Name: Rheb

Background: Rheb Antibody: Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Gene ID: 6009

UniProt: Q15382

Pathways: RTK Signaling