Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
Specificity
This TLR6 antibody is predicted to have no cross-reactivity to other members of the TLR protein family.
Purification
TLR6 Antibody is affinity chromatography purified via peptide column.
Immunogen
TLR6 antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human TLR6. The immunogen is located within amino acids 160 - 210 of TLR6.
TLR6 antibody can be used for detection of TLR6 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
TLR6 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
TLR6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
TLR1 antibody, TLR16 antibody, CD286 antibody, TLR-6 antibody, toll-like receptor 1 family member A antibody, toll like receptor 6 antibody, toll-like receptor 6 antibody, TLR1A antibody, TLR6 antibody, Tlr6 antibody
Background
TLR6 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR6 was first identified as a close homolog of TLR1, sharing 69 % sequence identify. Like TLR1, TLR6 can form heterodimers with TLR2, and these TLR6:TLR2 dimers coordinate macrophage activation by Gram-positive bacteria and the yeast cell wall particle zymosan. Activation of these complexes not only initiates pro-inflammatory cascades, but also can lead to apoptotic responses.