TLR9 antibody (AA 840-890)

Catalog No. ABIN6990609

This anti-TLR9 antibody is a Rabbit Polyclonal antibody detecting TLR9 in WB, ELISA, ICC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for TLR9 antibody (AA 840-890) (ABIN6990609)

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-TLR9 Antibody

Binding Specificity: AA 840-890

Specificity: At least two isoforms of TLR9 are known to exist, this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.

Purification: TLR9 Antibody is affinity chromatography purified via peptide column.

Immunogen: TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9. The immunogen is located within amino acids 840 - 890 of TLR9.

Isotype: IgG

Application Details

Application Notes: TLR9 antibody can be used for detection of TLR9 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TLR9 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TLR9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Alternative Name: TLR9

Background: TLR9 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Molecular Weight: Predicted: 104, 111 kDa

Observed: 97 kDa

Gene ID: 54106

UniProt: Q9NR96

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades