DRAM antibody (N-Term)

Catalog No. ABIN6990614

The Rabbit Polyclonal anti-DRAM antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect DRAM in samples from Human, Mouse and Rat.

Quick Overview for DRAM antibody (N-Term) (ABIN6990614)

Target: DRAM (DRAM1) (DNA-Damage Regulated Autophagy Modulator 1 (DRAM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRAM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-DRAM Antibody

Binding Specificity: AA 30-80, N-Term

Purification: DRAM Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-DRAM antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human DRAM. The immunogen is located within amino acids 30-80 of DRAM.

Isotype: IgG

Application Details

Application Notes: WB: 1-2 μ,g/mL, IF: 20 μ,g/mL, IHC: 2-2.5 μ,g/mL.

Antibody validated: Western Blot in human mouse and rat samples, Immunofluorescence and Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DRAM Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DRAM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DRAM

Target: DRAM (DRAM1) (DNA-Damage Regulated Autophagy Modulator 1 (DRAM1))

Alternative Name: DRAM

Background: DRAM Antibody: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

Molecular Weight: Predicted: 14kD, 26kD

Observed: 14 kD, 26kD kDa

Gene ID: 55332

UniProt: Q8N682