KDM3A antibody (N-Term)

Catalog No. ABIN6990704

The Rabbit Polyclonal anti-KDM3A antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect KDM3A in samples from Human and Rat.

Quick Overview for KDM3A antibody (N-Term) (ABIN6990704)

Target: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KDM3A Antibody

Binding Specificity: AA 50-100, N-Term

Specificity: This antibody will not cross-react with JMJD1B or JMJD1C.

Purification: JMJD1A Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-JMJD1A antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human JMJD1A. The immunogen is located within amino acids 50-100 of JMJD1A.

Isotype: IgG

Application Details

Application Notes: WB: 1-2 μ,g/mL, IHC: 5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples, Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: JMJD1A Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: JMJD1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for KDM3A

Target: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Alternative Name: JMJD1A

Background: JMJD1A Antibody: The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist.

Molecular Weight: Predicted: 147kD

Observed: 147kD kDa

Gene ID: 55818

NCBI Accession: NP_060903

UniProt: Q9Y4C1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Warburg Effect