TIGAR antibody (AA 60-110)

Catalog No. ABIN6990719

The Rabbit Polyclonal anti-TIGAR antibody has been validated for ELISA, WB and IHC (p). It is suitable to detect TIGAR in samples from Human, Mouse and Rat.

Quick Overview for TIGAR antibody (AA 60-110) (ABIN6990719)

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGAR antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TIGAR Antibody

Binding Specificity: AA 60-110

Purification: TIGAR Antibody is affinity chromatography purified via peptide column.

Immunogen: TIGAR antibody was raised against a 18 amino acid synthetic peptide from near the center of human TIGAR. The immunogen is located within amino acids 60 - 110 of TIGAR.

Isotype: IgG

Application Details

Application Notes: TIGAR antibody can be used for detection of TIGAR by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TIGAR Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TIGAR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Alternative Name: TIGAR

Background: TIGAR Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death, loss of its functions contributes to the development of most cancers. Recent studies have demonstrated the ability of p53 to regulate the expression of several proteins involved in glycolysis and oxidative phosphorylation, such as TIGAR, SCO2, and phosphoglycerate mutase. TIGAR is a recently discovered protein that functions to regulate glycolysis and protect cells against oxidative stress. TIGAR is similar in structure to proteins in the phosphoglycerate mutase family, most notably 6-phosphofructo-2-kinase, suggesting TIGAR may function as a fructose bisphosphatase. Expression of TIGAR in transfected cells correlated with an inhibition of glycolysis and decreased levels of reactive oxygen species and p53-induced apoptosis, indicating that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses.

Gene ID: 57103

NCBI Accession: NP_065108

UniProt: Q9NQ88

Pathways: Warburg Effect