TIGAR antibody (AA 24-188)

Catalog No. ABIN7153207

Product Details anti-TIGAR Antibody

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Binding Specificity: AA 24-188

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGAR antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Fructose-2,6-bisphosphatase TIGAR protein (24-188AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Alternative Name: TIGAR (TIGAR Products)

Synonyms: AA793651 antibody, AI595337 antibody, C79710 antibody, C85509 antibody, Tigar antibody, Trp53 induced glycolysis repulatory phosphatase antibody, Tigar antibody

Background:

Background: Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (PubMed:19015259). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed:16839880, PubMed:22887998). Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed:16839880, PubMed:19713938, PubMed:23726973, PubMed:22887998, PubMed:23817040). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity (PubMed:23185017). In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage (By similarity). Plays a role in adult intestinal regeneration, contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation (PubMed:23726973). Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions (By similarity). Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner (PubMed:22887998). Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses (PubMed:25928429). Involved in intestinal tumor progression (PubMed:23726973).

Aliases: 6-bisphosphatase TIGAR antibody, C12ORF5 antibody, chromosome 12 open reading frame 5 antibody, FR2BP antibody, Fructose-2,6-bisphosphatase TIGAR antibody, Fructose-2,6-bisphosphate 2-phosphatase antibody, Probable fructose 2,6 bisphosphatase TIGAR antibody, Probable fructose-2 antibody, tigar antibody, TIGAR_HUMAN antibody, TP53 induced glycolysis and apoptosis regulator antibody, TP53 induced glycolysis regulatory phosphatase antibody, TP53-induced glycolysis and apoptosis regulator antibody, Transactivated by NS3TP2 protein antibody

UniProt: Q9NQ88

Pathways: Warburg Effect