ERN1 antibody (C-Term)

Catalog No. ABIN6990723

This anti-ERN1 antibody is a Rabbit Polyclonal antibody detecting ERN1 in WB, ELISA, IF, ICC and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for ERN1 antibody (C-Term) (ABIN6990723)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ERN1 Antibody

Binding Specificity: C-Term

Purification: IRE1p Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-IRE1p antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRE1P. The immunogen is located within the last 50 amino acids of IRE1p.

Isotype: IgG

Application Details

Application Notes: WB: 0.5-2 μ,g/mL, ICC: 1 μ,g/mL, IF: 2-20 μ,g/mL, IHC: 2 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in mouse samples, Immunofluorescence in human, mouse and rat samples, Immunohistochemistry in human and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IRE1p Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IRE1p antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: IRE1p

Background: IRE1p Antibody: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: Predicted: 110kD

Observed: 110kD kDa

Gene ID: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response