SIRT2 antibody (N-Term)

Catalog No. ABIN6990863

The Rabbit Polyclonal anti-SIRT2 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect SIRT2 in samples from Human, Rat and Mouse.

Quick Overview for SIRT2 antibody (N-Term) (ABIN6990863)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIRT2 Antibody

Binding Specificity: N-Term

Purification: SIRT2 Antibody is affinity chromatography purified via peptide column.

Immunogen: SIRT2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human SIRT2. The immunogen is located within the first 50 amino acids of SIRT2.

Isotype: IgG

Application Details

Application Notes: SIRT2 antibody can be used for detection of SIRT2 by Western blot at 2.5 - 5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SIRT2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SIRT2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2

Background: SIRT2 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

Gene ID: 22933

NCBI Accession: NP_036369

UniProt: Q8IXJ6