RIMS2 antibody (AA 250-300)

Catalog No. ABIN6990907

This Rabbit Polyclonal antibody specifically detects RIMS2 in ELISA, WB, IHC (p) and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for RIMS2 antibody (AA 250-300) (ABIN6990907)

Target: RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIMS2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-RIMS2 Antibody

Binding Specificity: AA 250-300

Specificity: Multiple isoforms of RIM2 are known to exist. This antibody is predicted to have no cross-reactivity to other Rim proteins.

Purification: Rim2 Antibody is affinity chromatography purified via peptide column.

Immunogen: Rim2 antibody was raised against a 17 amino acid synthetic peptide near the center of the human Rim2. The immunogen is located within amino acids 250 - 300 of Rim2.

Isotype: IgG

Application Details

Application Notes: Rim2 antibody can be used for detection of Rim2 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rim2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Rim2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for RIMS2

Target: RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))

Alternative Name: Rim2

Background: Rim2 Antibody: Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. Rim1 and Rim2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release, synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like Rim1, Rim 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner.

Molecular Weight: Predicted: 39, 128, 148, 170 kDa

Observed: 128 kDa

Gene ID: 9699

NCBI Accession: NP_001093587

UniProt: Q9UQ26

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis