At least three isoforms of SAPAP2 are known to exist. This SAPAP2 antibody will not cross-react with other SAPAP proteins.
Purification
SAPAP2 Antibody is affinity chromatography purified via peptide column.
Immunogen
SAPAP2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human SAPAP2. The immunogen is located within amino acids 480 - 530 of SAPAP2.
DLGAP2
Reactivity: Human, Rat
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 488
Application Notes
SAPAP2 antibody can be used for detection of SAPAP2 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SAPAP2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
SAPAP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
DLGAP2
(Discs, Large (Drosophila) Homolog-Associated Protein 2 (DLGAP2))
PSD-95 antibody, DAP2 antibody, SAPAP2 antibody, 6430596N04Rik antibody, Sapap2 antibody, DLG associated protein 2 antibody, disks large-associated protein 2 antibody, discs, large (Drosophila) homolog-associated protein 2a antibody, discs, large (Drosophila) homolog-associated protein 2 antibody, DLGAP2 antibody, LOC463960 antibody, dlgap2a antibody, LOC100447448 antibody, Dlgap2 antibody
Background
SAPAP2 Antibody: SAP90/PSD-95-associated protein 2 (SAPAP2, also known as DLGAP2) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP2 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP2 mRNA is targeted to cell bodies in a similar manner to SAPAP1 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies.