NANOS3 antibody (C-Term)

Catalog No. ABIN6990927

Product Details anti-NANOS3 Antibody

Target: NANOS3 (Nanos Homolog 3 (NANOS3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NANOS3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.

Purification: Nanos3 Antibody is affinity chromatography purified via peptide column.

Immunogen: Nanos3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human Nanos3. The immunogen is located within the last 50 amino acids of Nanos3.

Isotype: IgG

Application Details

Application Notes: Nanos3 antibody can be used for detection of Nanos3 by Western blot at 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Nanos3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Nanos3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for NANOS3

Target: NANOS3 (Nanos Homolog 3 (NANOS3))

Alternative Name: Nanos3 (NANOS3 Products)

Synonyms: NANOS1L antibody, NOS3 antibody, nos3 antibody, cb725 antibody, nanos antibody, nanos1 antibody, nos1 antibody, nanos C2HC-type zinc finger 3 antibody, nanos homolog 3 (Drosophila) antibody, nanos homolog 3 antibody, NANOS3 antibody, Nanos3 antibody, nanos3 antibody

Background: Nanos3 Antibody: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.

Molecular Weight: Predicted: 19 kDa

Observed: 20 kDa

Gene ID: 342977

UniProt: P60323

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling