MED4 antibody (C-Term)

Catalog No. ABIN6990949

This Rabbit Polyclonal antibody specifically detects MED4 in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for MED4 antibody (C-Term) (ABIN6990949)

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MED4 Antibody

Binding Specificity: C-Term

Specificity: At least three isoforms of MED4 are known to exist, this antibody only recognizes the largest isoform.

Purification: MED4 Antibody is affinity chromatography purified via peptide column.

Immunogen: MED4 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MED4. The immunogen is located within the last 50 amino acids of MED4.

Isotype: IgG

Application Details

Application Notes: MED4 antibody can be used for detection of MED4 by Western blot at 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MED4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MED4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MED4

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Alternative Name: MED4

Background: MED4 Antibody: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment.

Gene ID: 29079

NCBI Accession: NP_054885

UniProt: Q9NPJ6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha