CNOT4 antibody (N-Term)

Catalog No. ABIN6990985

This Rabbit Polyclonal antibody specifically detects CNOT4 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CNOT4 antibody (N-Term) (ABIN6990985)

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CNOT4 Antibody

Binding Specificity: AA 70-120, N-Term

Purification: CNOT4 Antibody is affinity chromatography purified via peptide column.

Immunogen: CNOT4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CNOT4. The immunogen is located within amino acids 70 - 120 of CNOT4.

Isotype: IgG

Application Details

Application Notes: CNOT4 antibody can be used for detection of CNOT4 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CNOT4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CNOT4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CNOT4

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Alternative Name: CNOT4

Background: CNOT4 Antibody: CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.

Gene ID: 4850

NCBI Accession: NP_037448

UniProt: O95628