Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
NIPSNAP2 Antibody is affinity chromatography purified via peptide column.
Immunogen
NIPSNAP2 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human NIPSNAP2. The immunogen is located within the first 50 amino acids of NIPSNAP2.
NIPSNAP2 antibody can be used for detection of NIPSNAP2 by Western blot at 0.5 and 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
NIPSNAP2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
NIPSNAP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
NIPSNAP2 Antibody: The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40 % of glioblastomas, the most common and malignant form of central nervous system tumors. It is widely expressed and most abundant in heart and skeletal muscle. NIPSNAP proteins have been suggested to be important in vesicular transport. NIPSNAP2 antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.