GLE1 antibody (AA 390-440)

Catalog No. ABIN6991041

This anti-GLE1 antibody is a Rabbit Polyclonal antibody detecting GLE1 in WB, ELISA, IF and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for GLE1 antibody (AA 390-440) (ABIN6991041)

Target: GLE1 (GLE1 RNA Export Mediator (GLE1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GLE1 Antibody

Binding Specificity: AA 390-440

Purification: Gle1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Gle1 antibody was raised against a 14 amino acid synthetic peptide near the center of human Gle1. The immunogen is located within amino acids 390 - 440 of Gle1.

Isotype: IgG

Application Details

Application Notes: Gle1 antibody can be used for detection of Gle1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Gle1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Gle1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for GLE1

Target: GLE1 (GLE1 RNA Export Mediator (GLE1))

Alternative Name: Gle1

Background: Gle1 Antibody: The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.

Gene ID: 2733

NCBI Accession: NP_001003722

UniProt: Q53GS7