RNF168 antibody (C-Term)

Catalog No. ABIN6991108

Product Details anti-RNF168 Antibody

Target: RNF168 (Ring Finger Protein 168 (RNF168))

Binding Specificity: AA 440-490, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF168 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: RNF168 Antibody is affinity chromatography purified via peptide column.

Immunogen: RNF168 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human RNF168. The immunogen is located within amino acids 440 - 490 of RNF168.

Isotype: IgG

Application Details

Application Notes: RNF168 antibody can be used for detection of RNF168 by Western blot at 1 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RNF168 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RNF168 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for RNF168

Target: RNF168 (Ring Finger Protein 168 (RNF168))

Alternative Name: RNF168 (RNF168 Products)

Synonyms: 3110001H15Rik antibody, hRNF168 antibody, ring finger protein 168 antibody, ring finger protein 168, E3 ubiquitin protein ligase L homeolog antibody, Rnf168 antibody, rnf168.L antibody, RNF168 antibody

Background: RNF168 Antibody: RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.

Gene ID: 165918

NCBI Accession: NP_689830

UniProt: Q8IYW5

Pathways: Production of Molecular Mediator of Immune Response