SIGLEC11 antibody (C-Term)

Catalog No. ABIN6991110

Product Details anti-SIGLEC11 Antibody

Target: SIGLEC11 (Sialic Acid Binding Ig-Like Lectin 11 (SIGLEC11))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC11 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: Siglec11 Antibody is affinity chromatography purified via peptide column.

Immunogen: Siglec11 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human Siglec11. The immunogen is located within the last 50 amino acids of Siglec11.

Isotype: IgG

Application Details

Application Notes: Siglec11 antibody can be used for detection of Siglec11 by Western blot at 1 μ,g/mL. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Siglec11 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Siglec11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SIGLEC11

Target: SIGLEC11 (Sialic Acid Binding Ig-Like Lectin 11 (SIGLEC11))

Alternative Name: Siglec11 (SIGLEC11 Products)

Synonyms: sialic acid binding Ig like lectin 11 antibody, SIGLEC11 antibody

Background: Siglec11 Antibody: Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to alpha2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist. Siglec11 antibody will not cross-react with Siglec10.

Gene ID: 114132

NCBI Accession: NP_443116

UniProt: Q96RL6