Occludin antibody (C-Term)

Catalog No. ABIN6991125

This anti-Occludin antibody is a Rabbit Polyclonal antibody detecting Occludin in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Occludin antibody (C-Term) (ABIN6991125)

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Occludin Antibody

Binding Specificity: C-Term

Specificity: At least three isoforms of OCLN are known to exist.

Purification: OCLN Antibody is affinity chromatography purified via peptide column.

Immunogen: OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN. The immunogen is located within the last 50 amino acids of OCLN.

Isotype: IgG

Application Details

Application Notes: OCLN antibody can be used for detection of OCLN by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: OCLN Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: OCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN

Background: OCLN Antibody: Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.

Molecular Weight: Predicted: 57 kDa

Observed: 57 kDa

Gene ID: 4950

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C