ADAM10 antibody (C-Term)

Catalog No. ABIN6991127

The Rabbit Polyclonal anti-ADAM10 antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect ADAM10 in samples from Human.

Quick Overview for ADAM10 antibody (C-Term) (ABIN6991127)

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ADAM10 Antibody

Binding Specificity: C-Term

Purification: ADAM10 Antibody is affinity chromatography purified via peptide column.

Immunogen: ADAM10 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ADAM10. The immunogen is located within the last 50 amino acids of ADAM10.

Isotype: IgG

Application Details

Application Notes: ADAM10 can be used for detection of ADAM10 by Western blot at 1 - 2 μ,g/mL. This polyclonal antibody can also detect ADAM10 by immunohistochemistry at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ADAM10 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ADAM10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: ADAM10

Background: ADAM10 Antibody: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α,) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Molecular Weight: 85 kDa

Gene ID: 102

NCBI Accession: NP_001101

UniProt: O14672

Pathways: Notch Signaling, EGFR Signaling Pathway