THEM4 antibody (AA 60-110)

Catalog No. ABIN6991139

The Rabbit Polyclonal anti-THEM4 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect THEM4 in samples from Human, Mouse and Rat.

Quick Overview for THEM4 antibody (AA 60-110) (ABIN6991139)

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THEM4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-THEM4 Antibody

Binding Specificity: AA 60-110

Specificity: The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

Purification: THEM4 Antibody is affinity chromatography purified via peptide column.

Immunogen: THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4. The immunogen is located within amino acids 60 - 110 of THEM4.

Isotype: IgG

Application Details

Application Notes: THEM4 antibody can be used for detection of THEM4 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: THEM4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: THEM4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for THEM4

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Alternative Name: THEM4

Background: THEM4 Antibody: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.

Gene ID: 117145

NCBI Accession: NP_444283

UniProt: Q5T1C6

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway