SYPL2 antibody (C-Term)

Catalog No. ABIN6991151

The Rabbit Polyclonal anti-SYPL2 antibody is suitable to detect SYPL2 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IHC (p) and IF.

Quick Overview for SYPL2 antibody (C-Term) (ABIN6991151)

Target: SYPL2 (Synaptophysin-Like 2 (SYPL2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYPL2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-SYPL2 Antibody

Binding Specificity: C-Term

Specificity: At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1.

Purification: SYPL2 Antibody is affinity chromatography purified via peptide column.

Immunogen: SYPL2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SYPL. The immunogen is located within the last 50 amino acids of SYPL2.

Isotype: IgG

Application Details

Application Notes: SYPL2 antibody can be used for detection of SYPL2 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SYPL2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SYPL2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SYPL2

Target: SYPL2 (Synaptophysin-Like 2 (SYPL2))

Alternative Name: SYPL2

Background: SYPL2 Antibody: SYPL2, also known as Mitsugumin 29, was initially identified as a transmembrane protein from the triad junction in skeletal muscle that had significant homology with members of the synaptophysin family. SYPL2 is thought to participate in the excitation-contraction coupling process of skeletal muscle as SYPL2-null mice showed reduced muscle contractile force and altered triad junction structure and increased susceptibility to fatigue of the skeletal muscle. SYPL2 plays a critical role in muscle Ca2+ signaling by regulating the process of store-operated Ca2+ entry and interacts with ryanodine receptor (RyR), thereby influencing intracellular Ca2+ homeostasis through changes in the RyR/Ca2+ release function. Co-expression of SYPL2 and RyR in cultured cells leads to apoptotic cell death resulting from the depletion of Ca2+ from the intracellular stores.

Gene ID: 284612

NCBI Accession: NP_001035799

UniProt: Q5VXT5