MAK10/NAA35 antibody (AA 420-470)

Catalog No. ABIN6991160

Product Details anti-MAK10/NAA35 Antibody

Target: MAK10/NAA35 (MAK10) (MAK10 Homolog, Amino-Acid N-Acetyltransferase Subunit (MAK10))

Binding Specificity: AA 420-470

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAK10/NAA35 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: MAK10 Antibody is affinity chromatography purified via peptide column.

Immunogen: MAK10 antibody was raised against a 14 amino acid synthetic peptide near the center of human MAK10. The immunogen is located within amino acids 420 - 470 of MAK10.

Isotype: IgG

Application Details

Application Notes: MAK10 antibody can be used for detection of MAK10 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MAK10 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MAK10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MAK10/NAA35

Target: MAK10/NAA35 (MAK10) (MAK10 Homolog, Amino-Acid N-Acetyltransferase Subunit (MAK10))

Alternative Name: MAK10 (MAK10 Products)

Synonyms: MAK10 antibody, bA379P1.1 antibody, A330021G12Rik antibody, A330027C19Rik antibody, AI158944 antibody, C030004C14Rik antibody, Mak10 antibody, T4a antibody, mak10 antibody, wu:fb21g10 antibody, zgc:64157 antibody, N(alpha)-acetyltransferase 35, NatC auxiliary subunit antibody, NAA35 antibody, Naa35 antibody, naa35 antibody

Background: MAK10 Antibody: The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.

Gene ID: 60560

NCBI Accession: NP_078911

UniProt: Q5VZE5