IRGC antibody (C-Term)

Catalog No. ABIN6991167

This anti-IRGC antibody is a Rabbit Polyclonal antibody detecting IRGC in WB, ELISA, ICC and IF. Suitable for Human, Rat and Mouse.

Quick Overview for IRGC antibody (C-Term) (ABIN6991167)

Target: IRGC (Immunity-Related GTPase Family, Cinema (IRGC))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRGC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-IRGC Antibody

Binding Specificity: C-Term

Specificity: Two isoforms of IRGC are known to exist, this antibody will recognize both isoforms.

Purification: IRGC Antibody is affinity chromatography purified via peptide column.

Immunogen: IRGC antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human IRGC. The immunogen is located within the last 50 amino acids of IRGC.

Isotype: IgG

Application Details

Application Notes: IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IRGC Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IRGC antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IRGC

Target: IRGC (Immunity-Related GTPase Family, Cinema (IRGC))

Alternative Name: IRGC

Background: IRGC Antibody: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.

Gene ID: 56269

NCBI Accession: NP_062558

UniProt: Q6NXR0