KDM3B antibody (AA 1050-1100)

Catalog No. ABIN6991177

Product Details anti-KDM3B Antibody

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Binding Specificity: AA 1050-1100

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: This antibody will not cross-react with JMJD1A or JMJD1C.

Purification: JMJD1B Antibody is affinity chromatography purified via peptide column.

Immunogen: JMJD1B antibody was raised against a 20 amino acid synthetic peptide from near the center of human JMJD1B. The immunogen is located within amino acids 1050 - 1100 of JMJD1B.

Isotype: IgG

Application Details

Application Notes: JMJD1B antibody can be used for detection of JMJD1B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: JMJD1B Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: JMJD1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for KDM3B

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Alternative Name: JMJD1B (KDM3B Products)

Background: JMJD1B Antibody: The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist.

Gene ID: 51780

UniProt: Q7LBC6

Pathways: Warburg Effect