JMJD1C antibody (N-Term)

Catalog No. ABIN6991178

The Rabbit Polyclonal anti-JMJD1C antibody has been validated for ELISA, WB, IHC (p) and IF. It is suitable to detect JMJD1C in samples from Human, Mouse and Rat.

Quick Overview for JMJD1C antibody (N-Term) (ABIN6991178)

Target: JMJD1C (Jumonji Domain Containing 1C (JMJD1C))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JMJD1C antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-JMJD1C Antibody

Binding Specificity: AA 380-430, N-Term

Specificity: This antibody will not cross-react with JMJD1A or JMJD1B.

Purification: JMJD1C Antibody is affinity chromatography purified via peptide column.

Immunogen: JMJD1C antibody was raised against a 20 amino acid synthetic peptide from near the amino terminus of human JMJD1C. The immunogen is located within amino acids 380 - 430 of JMJD1C.

Isotype: IgG

Application Details

Application Notes: JMJD1C antibody can be used for detection of JMJD1C by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: JMJD1C Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: JMJD1C antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for JMJD1C

Target: JMJD1C (Jumonji Domain Containing 1C (JMJD1C))

Alternative Name: JMJD1C

Background: JMJD1C Antibody: The jumonji domain containing 1C protein (JMJD1C) was initially discovered in silico, and later suggested to be a candidate gene for autism. Like the related proteins JMJD1A and JMJD1B, JMJD1C is a histone H3K9 demethylase implicated in the nuclear hormone receptor-based transcriptional regulation. JMJD1C mRNA is highly expressed in undifferentiated embryonic stem (ES) cells as well as pancreatic islet, diffuse-type gastric cancer, and other tissues and tumors. The JMJD1C gene promoter contain bHLH-, AP-1-, and POU5F1-binding sites, and as preferential expression of POU5F1 has been reported in ES cells, pancreatic islet, and diffuse-type gastric cancer, it has been suggested that POU5F1-mediated expression of JMJD1C reactivates previously silenced genes in ES cells and diffuse-type gastric cancer. At least three isoforms of JMJD1C are known to exist.

Gene ID: 221037

NCBI Accession: NP_116165

UniProt: Q15652

Pathways: Nuclear Hormone Receptor Binding