ZNF536 antibody (N-Term)

Catalog No. ABIN6991232

The Rabbit Polyclonal anti-ZNF536 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect ZNF536 in samples from Human, Mouse and Rat.

Quick Overview for ZNF536 antibody (N-Term) (ABIN6991232)

Target: ZNF536 (Zinc Finger Protein 536 (ZNF536))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF536 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-ZNF536 Antibody

Binding Specificity: AA 30-80, N-Term

Purification: ZNF536 Antibody is affinity chromatography purified via peptide column.

Immunogen: ZNF536 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ZNF536. The immunogen is located within amino acids 30 - 80 of ZNF536.

Isotype: IgG

Application Details

Application Notes: ZNF536 antibody can be used for detection of ZNF536 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZNF536 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZNF536 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZNF536

Target: ZNF536 (Zinc Finger Protein 536 (ZNF536))

Alternative Name: ZNF536

Background: ZNF536 Antibody: ZNF536 is a recently identified zinc-finger protein that is expressed primarily in the developing nervous system and the cerebral cortex, hippocampus, and hypothalamus. ZNF536 possess ten zinc fingers and interacts with CtBP1, a corepressor for gene transcription. It is most closely related to transcriptional repressor ZNF219. Overexpression of ZNF536 in embryonic stem cells dramatically reduced the mRNA levels of neuronal marker genes such as Pax6, MAP2, and beta-tubulin III following retinoic acid (RA)-induced differentiation, while depletion of ZNF536 via RNAi resulted in elevated mRNA levels of these genes, indicating its role in inhibiting neuronal cell differentiation. Overexpression of RA receptor a rescues the inhibitory role of ZNF536, suggesting that ZNF536 might inhibit RA response element-mediated transcriptional activity.

Gene ID: 9745

NCBI Accession: NP_055532

UniProt: O15090

Pathways: Retinoic Acid Receptor Signaling Pathway