PKDCC antibody (AA 320-370)

Catalog No. ABIN6991253

The Rabbit Polyclonal anti-PKDCC antibody is suitable to detect PKDCC in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for PKDCC antibody (AA 320-370) (ABIN6991253)

Target: PKDCC (Protein Kinase Domain Containing, Cytoplasmic Homolog (Mouse) (PKDCC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKDCC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKDCC Antibody

Binding Specificity: AA 320-370

Purification: VLK Antibody is affinity chromatography purified via peptide column.

Immunogen: VLK antibody was raised against a 15 amino acid synthetic peptide from near the center of human VLK. The immunogen is located within amino acids 320 - 370 of VLK.

Isotype: IgG

Application Details

Application Notes: VLK antibody can be used for detection of VLK by Western blot at 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: VLK Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: VLK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PKDCC

Target: PKDCC (Protein Kinase Domain Containing, Cytoplasmic Homolog (Mouse) (PKDCC))

Alternative Name: VLK

Background: VLK Antibody: VLK was identified as a novel protein kinase that was induced after the differentiation of cultured embryonic stem cells into mesendoderm. It has no homologs in invertebrates, but is highly conserved in vertebrate species although it does not belong to any known protein kinase groups. VLK is initially expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, but its expression is later confined to E-cadherin-negative mesenchyme. It is enriched in the Golgi apparatus and is thought to regulate the rate of protein export from the Golgi. Targeted disruption of VLK in mice leads to a defect in lung development and neonatal lethality. It has been suggested that mutations in VLK may be associated with the allergic condition atopy.

Molecular Weight: Predicted: 32, 54 kDa

Observed: 31, 55 kDa 

Gene ID: 91461

NCBI Accession: NP_612379

UniProt: Q5RJI4