ATG13 antibody (C-Term)

Catalog No. ABIN6991353

This anti-ATG13 antibody is a Rabbit Polyclonal antibody detecting ATG13 in WB, ELISA, IHC (p) and IF. Suitable for Human, Mouse and Rat.

Quick Overview for ATG13 antibody (C-Term) (ABIN6991353)

Target: ATG13 (Autophagy Related 13 (ATG13))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-ATG13 Antibody

Binding Specificity: AA 450-500, C-Term

Specificity: Multiple isoforms of ATG13 are known to exist.

Purification: ATG13 Antibody is affinity chromatography purified via peptide column.

Immunogen: ATG13 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATG13. The immunogen is located within amino acids 450 - 500 of ATG13.

Isotype: IgG

Application Details

Application Notes: ATG13 antibody can be used for detection of ATG13 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ATG13 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ATG13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATG13

Target: ATG13 (Autophagy Related 13 (ATG13))

Alternative Name: ATG13

Background: ATG13 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions, inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.

Molecular Weight: Predicted: 44, 53, 57, 61 kDa

Observed: 49 kDa

Gene ID: 9776

NCBI Accession: NP_001136145

UniProt: O75143

Pathways: PI3K-Akt Signaling, Autophagy