ZEB1 antibody (AA 530-580)

Catalog No. ABIN6991365

This anti-ZEB1 antibody is a Rabbit Polyclonal antibody detecting ZEB1 in WB, ELISA, IF, ICC and IHC (p). Suitable for Human.

Quick Overview for ZEB1 antibody (AA 530-580) (ABIN6991365)

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ZEB1 Antibody

Binding Specificity: AA 530-580

Specificity: Multiple isoforms of ZEB1 are known to exist.

Purification: ZEB1 Antibody is affinity chromatography purified via peptide column.

Immunogen: ZEB1 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1. The immunogen is located within amino acids 530 - 580 of ZEB1.

Isotype: IgG

Application Details

Application Notes: ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 20 μ,g/mL and immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZEB1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZEB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1

Background: ZEB1 Antibody: ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.

Molecular Weight: Predicted: 124 kDa

Observed: 130 kDa

Gene ID: 6935

NCBI Accession: NP_110378

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation