SCUBE3 antibody (AA 440-490)

Catalog No. ABIN6991375

This Rabbit Polyclonal antibody specifically detects SCUBE3 in ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SCUBE3 antibody (AA 440-490) (ABIN6991375)

Target: SCUBE3 (Signal Peptide, CUB Domain, EGF-Like 3 (SCUBE3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCUBE3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SCUBE3 Antibody

Binding Specificity: AA 440-490

Purification: SCUBE3 Antibody is affinity chromatography purified via peptide column.

Immunogen: SCUBE3 antibody was raised against a 15 amino acid peptide near the center of human SCUBE3. The immunogen is located within amino acids 440 - 490 of SCUBE3.

Isotype: IgG

Application Details

Application Notes: SCUBE3 antibody can be used for detection of SCUBE3 by immunohistochemistry at 5 μ,g/mL.

Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SCUBE3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SCUBE3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for SCUBE3

Target: SCUBE3 (Signal Peptide, CUB Domain, EGF-Like 3 (SCUBE3))

Alternative Name: SCUBE3

Background: SCUBE3 Antibody: SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart (1,2). Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth. SCUBE3 has been shown to be an endogenous TGF-beta receptor ligand (3,4) and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-beta1, VEGF, Snail, and Slug.

Molecular Weight: Predicted: 109 kDa

Gene ID: 222663

NCBI Accession: NP_689966

UniProt: Q8IX30