Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
At least three isoforms of ZMYM3 are known to exist, this antibody will detect all three. ZMYM3 antibody is predicted to not cross-react with other ZMYM protein family members.
Purification
ZMYM3 Antibody is affinity chromatography purified via peptide column.
Immunogen
ZMYM3 antibody was raised against a 18 amino acid synthetic peptide near the center of human ZMYM3. The immunogen is located within amino acids 220 - 270 of ZMYM3.
ZMYM3
Reactivity: Human
WB, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ZMYM3 antibody can be used for detection of ZMYM3 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
ZMYM3 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
ZMYM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ZMYM3 Antibody: Zinc-finger proteins contain DNA-binding domains characterized by the unique role of zinc and have a wide variety of functions such as transcriptional activation or repression. The protein folding and the DNA binding ability are governed by the coordination of a zinc ion. As a member of the MYM (myeloproliferative and mental retardation) gene family, ZMYM3 is highly conserved in vertebrates and most abundantly expressed in the brain. The encoded protein is a component of histone deacetylase-containing multiprotein complexes that function through modifying chromatin structure to keep genes silent. A chromosomal translocation (X,13) involving this gene is associated with X-linked mental retardation.