ESRP1 antibody (N-Term)

Catalog No. ABIN6991490

The Rabbit Polyclonal anti-ESRP1 antibody is suitable to detect ESRP1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p) and IF.

Quick Overview for ESRP1 antibody (N-Term) (ABIN6991490)

Target: ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESRP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-ESRP1 Antibody

Binding Specificity: AA 30-80, N-Term

Specificity: RBM35A antibody is predicted to not cross-react with other RBM35/ESRP family members. At least five isoforms of RBM35A are known to exist, this antibody will detect all five.

Purification: RBM35A Antibody is affinity chromatography purified via peptide column.

Immunogen: RBM35A antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RBM35A. The immunogen is located within amino acids 30 - 80 of RBM35A.

Isotype: IgG

Application Details

Application Notes: RBM35A antibody can be used for detection of RBM35A by Western blot at 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RBM35A Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RBM35A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ESRP1

Target: ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))

Alternative Name: RBM35A

Background: RBM35A Antibody: RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.

Gene ID: 54845

NCBI Accession: NP_001030087

UniProt: Q6NXG1