ZNF281 antibody (C-Term)

Catalog No. ABIN6991506

Product Details anti-ZNF281 Antibody

Target: ZNF281 (Zinc Finger Protein 281 (ZNF281))

Binding Specificity: AA 600-650, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF281 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purification: ZFP281 Antibody is affinity chromatography purified via peptide column.

Immunogen: ZFP281 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFP281. The immunogen is located within amino acids 600 - 650 of ZFP281.

Isotype: IgG

Application Details

Application Notes: ZFP281 antibody can be used for detection of ZFP281 by Western blot at 0.5 μ,g/mL. Antibody can also be used for immunofluorescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZFP281 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZFP281 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZNF281

Target: ZNF281 (Zinc Finger Protein 281 (ZNF281))

Alternative Name: ZFP281 (ZNF281 Products)

Background: ZFP281 Antibody: ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.

Gene ID: 23528

UniProt: Q9Y2X9

Pathways: Embryonic Body Morphogenesis