DDA (C-Term) antibody

Catalog No. ABIN6991579

This Rabbit Polyclonal antibody specifically detects DDA in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DDA (C-Term) antibody (ABIN6991579)

Target: DDA

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: C-Term

Purification: DDA Antibody is affinity chromatography purified via peptide column.

Immunogen: DDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DDA1. The immunogen is located within the last 50 amino acids of DDA.

Isotype: IgG

Application Details

Application Notes: DDA1 antibody can be used for detection of DDA1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DDA Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DDA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details

Target: DDA

Background: DDA Antibody: DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.

Gene ID: 79016

NCBI Accession: NP_003253

UniProt: Q9BW61