HIGD1A antibody (N-Term)

Catalog No. ABIN6991582

This Rabbit Polyclonal antibody specifically detects HIGD1A in WB, IF, ELISA and ICC. It exhibits reactivity toward Human.

Quick Overview for HIGD1A antibody (N-Term) (ABIN6991582)

Target: HIGD1A (HIG1 Hypoxia Inducible Domain Family, Member 1A (HIGD1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIGD1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-HIGD1A Antibody

Binding Specificity: N-Term

Specificity: HIG1 antibody is predicted to not cross-react with HIG2

Purification: HIG1 Antibody is affinity chromatography purified via peptide column.

Immunogen: HIG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HIG1. The immunogen is located within the first 50 amino acids of HIG1.

Isotype: IgG

Application Details

Application Notes: HIG1 antibody can be used for detection of HIG1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: HIG1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: HIG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for HIGD1A

Target: HIGD1A (HIG1 Hypoxia Inducible Domain Family, Member 1A (HIGD1A))

Alternative Name: HIG1

Background: HIG1 Antibody: HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG1 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis.

Gene ID: 25994

NCBI Accession: NP_001093138

UniProt: Q9Y241