PRICKLE2 antibody (AA 420-470)

Catalog No. ABIN6991607

Product Details anti-PRICKLE2 Antibody

Target: PRICKLE2 (Prickle Homolog 2 (PRICKLE2))

Binding Specificity: AA 420-470

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRICKLE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Two isoforms of PRICKLE2 are known to exist. PRICKLE2 antibody is predicted to not cross-react with other PRICKLE protein family members.

Purification: PRICKLE2 Antibody is affinity chromatography purified via peptide column.

Immunogen: PRICKLE2 antibody was raised against a 15 amino acid synthetic peptide near the center of human PRICKLE2. The immunogen is located within amino acids 420 - 470 of PRICKLE2.

Isotype: IgG

Application Details

Application Notes: PRICKLE2 antibody can be used for detection of PRICKLE2 by Western blot at 1 μ,g/mL. Antibody can also be used for immunofluorescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PRICKLE2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PRICKLE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PRICKLE2

Target: PRICKLE2 (Prickle Homolog 2 (PRICKLE2))

Alternative Name: PRICKLE2 (PRICKLE2 Products)

Synonyms: EPM5 antibody, 6230400G14Rik antibody, 6720451F06Rik antibody, mpk2 antibody, cb886 antibody, im:7138215 antibody, pk2 antibody, prickle planar cell polarity protein 2 antibody, prickle homolog 2b antibody, PRICKLE2 antibody, Prickle2 antibody, prickle2b antibody

Background: PRICKLE2 Antibody: PRICKLE2, also known as Pk2 or EPM5, is a member of a highly conserved protein family that function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity. Both PRICKLE2 and the related protein PRICKLE1 are expressed in postmitotic neurons and promote neurite outgrowth, and both proteins promote neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. PRICKLE2 localizes to the postsynaptic density and interacts with PSD-95 and NMDA receptors. Defects in the gene encoding PRICKLE2 are associated with autosomal recessive progressive myoclonic epilepsy.

Gene ID: 166336

NCBI Accession: NP_942559

UniProt: Q7Z3G6