PIWIL2 antibody (AA 410-460)

Catalog No. ABIN6991609

This Rabbit Polyclonal antibody specifically detects PIWIL2 in WB, ELISA, ICC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for PIWIL2 antibody (AA 410-460) (ABIN6991609)

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIWIL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PIWIL2 Antibody

Binding Specificity: AA 410-460

Specificity: At least three isoforms of PIWI-L2 are known to exist, this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members.

Purification: PIWI-L2 Antibody is affinity chromatography purified via peptide column.

Immunogen: PIWI-L2 antibody was raised against a 17 amino acid synthetic peptide near the center of human PIWI-L2. The immunogen is located within amino acids 410 - 460 of PIWI-L2.

Isotype: IgG

Application Details

Application Notes: PIWI-L2 antibody can be used for detection of PIWI-L2 by Western blot at 1 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PIWI-L2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PIWI-L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PIWIL2

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Alternative Name: PIWI-L2

Background: PIWI-L2 Antibody: PIWI-L2 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L2 has been implicated in multiple roles including stem cell self-renewal and is highly expressed in many breast cancers. In these tumors, PIWI-L2 silences the expression of STAT3, a regulator of Bcl-XL and cyclin D1, allowing the proliferation and survival of breast cancer stem cells. Recent studies have shown that overexpression of PIWI-like proteins are potential biomarkers for colon and other cancers.

Gene ID: 55124

NCBI Accession: NP_00129193

UniProt: Q8TC59

Pathways: Stem Cell Maintenance