Nkx2-2 antibody (AA 90-140)

Catalog No. ABIN6991653

This anti-Nkx2-2 antibody is a Rabbit Polyclonal antibody detecting Nkx2-2 in IF, IHC (p), WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for Nkx2-2 antibody (AA 90-140) (ABIN6991653)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA

Product Details anti-Nkx2-2 Antibody

Binding Specificity: AA 90-140

Specificity: NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members.

Purification: NKX2-2 Antibody is affinity chromatography purified via peptide column.

Immunogen: NKX2-2 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-2. The immunogen is located within amino acids 90 - 140 of NKX2-2.

Isotype: IgG

Application Details

Application Notes: NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NKX2-2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NKX2-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2-2

Background: NKX2-2 Antibody: NKX2-2 (NK2 homeobox 2) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-2 is crucial in the development of pancreatic islet cell differentiation and activates the ghrelin-promoter in these cells. NKX2-2 is expressed at the onset of pancreatic epithelium formation and becomes restricted to mature α,-, β,- and PP cells, and in NKX2-2-null mice embryos, all β,-cells and most a-cells are replaced by ghrelin-positive cells. NKX2-2 is also thought to be involved in ventral neuronal patterning and floor plate development and commissural axon guidance.

Gene ID: 4821

NCBI Accession: NP_002500

UniProt: O95096

Pathways: Dopaminergic Neurogenesis