SIGLEC15 antibody (N-Term)

Catalog No. ABIN6991662

Product Details anti-SIGLEC15 Antibody

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Binding Specificity: AA 50-100, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC15 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: SIGLEC15 antibody is predicted to not cross-react with other SIGLEC family members.

Purification: SIGLEC15 Antibody is affinity chromatography purified via peptide column.

Immunogen: SIGLEC15 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SIGLEC15. The immunogen is located within amino acids 50 - 100 of SIGLEC15.

Isotype: IgG

Application Details

Application Notes: SIGLEC15 antibody can be used for detection of SIGLEC15 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SIGLEC15 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SIGLEC15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SIGLEC15

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Alternative Name: SIGLEC15 (SIGLEC15 Products)

Background: SIGLEC15 Antibody: Siglecs are vertebrate cell-surface lectins that recognize sialylated glycans and are involved in many physiological processes, such as glycoprotein turnover, cellular trafficking, and pathogen recognition. Most Siglecs are expressed on cells of the immune system. SIGLEC15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail. SIGLEC15 can interact with the activating adaptor molecules DAP12/10. Its activating signaling potential and unique preference for glycan recognition implies that SIGLEC15 may be involved in the immune surveillance of tumors and probably plays a conserved, regulatory role in the immune system of vertebrates.

Molecular Weight: Predicted: 36 kDa

Observed: 42 kDa

Gene ID: 284266

NCBI Accession: NP_998767

UniProt: Q6ZMC9