PRC1 antibody (AA 350-400)

Catalog No. ABIN6991683

This anti-PRC1 antibody is a Rabbit Polyclonal antibody detecting PRC1 in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for PRC1 antibody (AA 350-400) (ABIN6991683)

Target: PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PRC1 Antibody

Binding Specificity: AA 350-400

Specificity: At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification: PRC1 Antibody is affinity chromatography purified via peptide column.

Immunogen: PRC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human PRC1. The immunogen is located within amino acids 350 - 400 of PRC1.

Isotype: IgG

Application Details

Application Notes: PRC1 antibody can be used for detection of Prc1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PRC1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PRC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PRC1

Target: PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))

Alternative Name: PRC1

Background: PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.

Gene ID: 9055

NCBI Accession: NP_003972

UniProt: O43663