CP110 antibody (C-Term)

Catalog No. ABIN6991685

The Rabbit Polyclonal anti-CP110 antibody is suitable to detect CP110 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p) and IF.

Quick Overview for CP110 antibody (C-Term) (ABIN6991685)

Target: CP110 (CCP110) (Centriolar Coiled Coil Protein 110kDa (CCP110))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CP110 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-CP110 Antibody

Binding Specificity: C-Term

Specificity: Two isoforms of CCP110 are known to exist, this antibody will only detect the longer isoform

Purification: CCP110 Antibody is affinity chromatography purified via peptide column.

Immunogen: CCP110 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human CCP110. The immunogen is located within the last 50 amino acids of CCP110.

Isotype: IgG

Application Details

Application Notes: CCP110 antibody can be used for detection of CCP110 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CCP110 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CCP110 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CP110

Target: CP110 (CCP110) (Centriolar Coiled Coil Protein 110kDa (CCP110))

Alternative Name: CCP110

Background: CCP110 Antibody: CCP110 is a centrosomal protein required for the centrosome to function as a microtubule organizing center and plays an essential role in centrosome duplication. It is a target of cyclin-dependent kinases (CDKs), disruption of CCP110 phosphorylation leads to unscheduled centrosome separation and polyploidy. CCP110 is also a target of SCF (Cyclin F) ubiquitin ligase complex, leading to its degradation, suggesting that this complex controls centrosome homeostasis and mitotic fidelity through CCP110 degradation. The centriolar kinesin Kif24 also interacts with CP110 and remodel microtubules and regulate ciliogenesis.

Molecular Weight: Predicted: 110 kDa

Observed: 110 kDa

Gene ID: 9738

NCBI Accession: NP_001185951

UniProt: O43303

Pathways: M Phase