CENPW antibody (N-Term)

Catalog No. ABIN6991719

The Rabbit Polyclonal anti-CENPW antibody (ABIN6991719) specifically detects CENPW in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CENPW antibody (N-Term) (ABIN6991719)

Target: CENPW (Centromere Protein W (CENPW))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPW antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CENPW Antibody

Binding Specificity: N-Term

Specificity: At least three isoforms of CENPW are known to exist, this antibody will detect all three isoforms.

Purification: CENPW Antibody is affinity chromatography purified via peptide column.

Immunogen: CENPW antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CENPW. The immunogen is located within the first 50 amino acids of CENPW.

Isotype: IgG

Application Details

Application Notes: CENPW antibody can be used for detection of CENPW by Western blot at 0.5 - 1 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CENPW Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CENPW antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CENPW

Target: CENPW (Centromere Protein W (CENPW))

Alternative Name: CENPW

Background: CENPW Antibody: CENPW was initially identified as a gene that was upregulated in multiple cancers, and whose overexpression in mouse fibroblast cells gave rise to distinct cancer-specific phenotypes. It was later found to be a nuclear protein that associated with CENPT, a component of CENPA nucleosome complex in the centromere, and is required for proper kinetochore function. CENPW also specifically interacts with the nucleolar phosphoprotein nucleophosmin, also known as B23 It has been suggested that nucleophosmin functions in the assembly of the kinetochore by interacting with CENPW during interphase. Overexpression of CENPW in the SKOV-3 human ovarian cancer cell line as well as in the zebrafish embryo led to apoptosis, suggesting that high levels of CENPW induces apoptotic cell death.

Gene ID: 387103

NCBI Accession: NP_001012525

UniProt: Q5EE01