BCAR3 antibody (N-Term)

Catalog No. ABIN6991737

The Rabbit Polyclonal anti-BCAR3 antibody (ABIN6991737) specifically detects BCAR3 in WB, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BCAR3 antibody (N-Term) (ABIN6991737)

Target: BCAR3 (Breast Cancer Anti-Estrogen Resistance 3 (BCAR3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAR3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-BCAR3 Antibody

Binding Specificity: AA 30-80, N-Term

Specificity: At least four isoforms of BCAR3 are known to exist, this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins.

Purification: BCAR3 Antibody is affinity chromatography purified via peptide column.

Immunogen: BCAR3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BCAR3. The immunogen is located within amino acids 30 - 80 of BCAR3.

Isotype: IgG

Application Details

Application Notes: BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: BCAR3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: BCAR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for BCAR3

Target: BCAR3 (Breast Cancer Anti-Estrogen Resistance 3 (BCAR3))

Alternative Name: BCAR3

Background: BCAR3 Antibody: Breast cancer anti-estrogen resistance 3 (BCAR3) was identified in the search for genes involved in the development of estrogen resistance. It contains a putative src homology 2 (SH2) domain and is partly homologous to the cell division cycle protein CDC48. BCAR3 is a binding partner with Cas, an adapter molecule that plays a role in cell proliferation, survival, cell adhesion, and mobility. BCAR3 functions synergistically with Cas to enhance Src activation and promote cell migration, and has been suggested to regulate Cas/Src association, Src kinase activity, and breast cancer adhesion signaling.

Molecular Weight: 91 kDa

Gene ID: 8412

NCBI Accession: NP_003558

UniProt: O75815