EED antibody (N-Term)

Catalog No. ABIN6991742

The Rabbit Polyclonal anti-EED antibody has been validated for WB, ELISA and IF. It is suitable to detect EED in samples from Human, Mouse and Rat.

Quick Overview for EED antibody (N-Term) (ABIN6991742)

Target: EED (Embryonic Ectoderm Development (EED))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-EED Antibody

Binding Specificity: AA 30-80, N-Term

Specificity: Four alternatively spliced transcript variants have been observed.

Purification: EED Antibody is affinity chromatography purified via peptide column.

Immunogen: EED antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EED. The immunogen is located within amino acids 30 - 80 of EED.

Isotype: IgG

Application Details

Application Notes: EED antibody can be used for detection of EED by Western blot at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: EED Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: EED antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED

Background: EED Antibody: The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin β,7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.

Molecular Weight: Predicted: 49 kDa

Observed: 49 kDa

Gene ID: 8726

NCBI Accession: NP_003788

UniProt: O75530