SHISA3 antibody (AA 120-170)

Catalog No. ABIN6991837

Product Details anti-SHISA3 Antibody

Target: SHISA3 (Shisa Homolog 3 (SHISA3))

Binding Specificity: AA 120-170

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHISA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: SHISA3 antibody is predicted to not cross-react with other SHISA protein family members.

Purification: SHISA3 Antibody is affinity chromatography purified via peptide column.

Immunogen: SHISA3 antibody was raised against a 15 amino acid peptide near the center of human SHISA3 . The immunogen is located within amino acids 120 - 170 of SHISA3.

Isotype: IgG

Application Details

Application Notes: SHISA3 Antibody can be used for detection of SHISA3 by Western blot at 1 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SHISA3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SHISA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for SHISA3

Target: SHISA3 (Shisa Homolog 3 (SHISA3))

Alternative Name: SHISA3 (SHISA3 Products)

Synonyms: hShisa3 antibody, D830007B15Rik antibody, shisa family member 3 antibody, SHISA3 antibody, Shisa3 antibody

Background: SHISA3 Antibody: SHISA3 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.

Molecular Weight: Predicted: 26 kDa

Observed: 28 kDa

Gene ID: 152573

NCBI Accession: NP_001073974

UniProt: A0PJX4