ULK1 antibody (AA 350-400)

Catalog No. ABIN6991858

This Rabbit Polyclonal antibody specifically detects ULK1 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ULK1 antibody (AA 350-400) (ABIN6991858)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ULK1 Antibody

Binding Specificity: AA 350-400

Specificity: Two alternatively spliced transcript variants encoding different isoforms have been identified. ULK1 antibody is predicted to not cross-react with ULK2.

Purification: ULK1 Antibody is affinity chromatography purified via peptide column.

Immunogen: ULK1 antibody was raised against a 16 amino acid peptide near the center of human ULK1 . The immunogen is located within amino acids 350 - 400 of ULK1.

Isotype: IgG

Application Details

Application Notes: ULK1 Antibody can be used for detection of ULK1 by Western blot at 1 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ULK1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ULK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Alternative Name: ULK1

Background: ULK1 Antibody: ULK1, also known as ATG1, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK1 results in a severe defect in the autophagy pathway. ULK1 is highly conserved among eukaryotes, and are the Unc-51-like kinases, ULK1 and ULK2 in mammals. ULK1 is ubiquitously expressed and involved in autophagy in response to starvation. It is the target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13. ULK1 also plays a role early in neuronal differentiation.

Molecular Weight: Predicted: 116 kDa

Observed: 105 kDa

Gene ID: 8408

NCBI Accession: NP_003556

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy