UNG antibody (N-Term)

Catalog No. ABIN6991863

This anti-UNG antibody is a Rabbit Polyclonal antibody detecting UNG in WB, ELISA and IF. Suitable for Human and Mouse.

Quick Overview for UNG antibody (N-Term) (ABIN6991863)

Target: UNG (Uracil-DNA Glycosylase (UNG))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-UNG Antibody

Binding Specificity: N-Term

Specificity: At least two isoforms of UNG2 are known to exist, this antibody will only detect the longer isoform. UNG2 antibody is predicted to not cross-react with UNG1.

Purification: UNG2 Antibody is affinity chromatography purified via peptide column.

Immunogen: UNG2 antibody was raised against a 17 amino acid peptide near the amino terminus of human UNG2. The immunogen is located within the first 50 amino acids of UNG2.

Isotype: IgG

Application Details

Application Notes: UNG2 antibody can be used for detection of UNG2 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: UNG2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: UNG2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for UNG

Target: UNG (Uracil-DNA Glycosylase (UNG))

Alternative Name: UNG2

Background: UNG2 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promoters and alternative splicing. UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from DNA that arise through either misincorporation during replication or cytosine deamination. UNG2 can also be bound by the HIV-1 integrase and incorporated into the virion particle, suggesting that it is required to remove uracils from the viral genome. As the intrinsic antiviral protein APOBEC3G generates numerous uracils in the HIV genome during its replication, it may be that the UNG2 contributes to the APOBEC3G-mediated loss of infectivity by generating abasic sites in the viral genome.

Molecular Weight: Predicted: 34 kDa

Observed: 33 kDa

Gene ID: 7374

NCBI Accession: NP_550433

UniProt: P13051

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response